A certificate of analysis (COA) is the document that shows what a laboratory found when it tested a specific batch of a compound. For research peptides, it is the main way to confirm three things before an experiment starts:
A study is only as reliable as the materials that go into it, so knowing how to read a COA is a basic research skill. Aion Aminos posts certificates of analysis on product pages so researchers can check this information before ordering.
The batch identifier is important. A COA describes one specific batch, so researchers should be able to match it to the material they receive.
High performance liquid chromatography (HPLC) is the most widely used method for measuring peptide purity. The sample is pushed through a column under high pressure. Different molecules travel through the column at different speeds and come out at different times, which are recorded as peaks on a chart called a chromatogram.
How to read it:
HPLC purity tells you how clean the sample is, but on its own it does not prove the main peak is the right molecule. That is where mass spectrometry helps.
Mass spectrometry (MS) measures the mass of molecules in a sample. Because every peptide has a known molecular weight, the measured mass is strong evidence of identity.
When a COA combines HPLC and MS (often as LC-MS), it shows both that the sample is pure and that the main component is the correct peptide.
Some laboratories use UV-Vis spectrophotometry, which measures how a sample absorbs ultraviolet and visible light. Results you may see include:
UV-Vis is fast and well suited to checking amount per vial. It is less able than HPLC to separate closely related impurities, and less specific than MS for identity, so it is useful to know which method produced each number.
The assay, or quantitative content, tells you how much peptide is actually in the vial. A typical specification is not less than 95% of the label claim. For example, a 10 mg vial with a measured content of 10.02 mg meets that specification.
Purity and assay are different measurements. A sample can be very pure but slightly under-filled, or correctly filled but with more impurities. A good COA reports both.
Results of 0 CFU, or values well below the stated limit, indicate the sample passed these checks.
Most COAs present each test in four columns: component, test, specification and result. Read across each row and ask:
Certificates of analysis are shown in the image gallery on each product page. For example, see BPC-157, Hexarelin Acetate or MOTS-c. If you need the COA for a specific batch, contact our team.
A peptide COA answers three questions: is it the right compound, how pure is it, and is the amount correct. HPLC is the standard tool for purity, mass spectrometry confirms identity, and UV-Vis is often used for identity screening and amount per vial. Reading the method, specification and result for each test, and checking the batch identifier, lets researchers judge the quality of their materials before work begins.
All Aion Aminos products are sold strictly for laboratory research use only. They are not intended for human or animal use and are not drugs, foods, supplements or cosmetics.
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